CLC Nuclear Magnetic Resonance (NMR) Core Facility

Services

  • Sample preparation
  • NMR data generation and analysis
    • Binding and association of biomolecules
    • Protein structural determination
    • Protein dynamics analysis
    • Chemical analysis
    • Metabolomics analysis
  • Consultation
  • Seminars, workshops and training

Summary

The WCM CLC Nuclear Magnetic Resonance (NMR) Core Facility provides state-of-the-art biological and chemical nuclear magnetic resonance resources and services, and expertise in their analytical, structural and biochemical applications, to the Weill Cornell Medicine (WCM) community and to outside investigators. Services include sample preparation, NMR data collection and data analysis. The core facility provides consultation on project design and data analysis, and offers seminars, training and educational workshops.

Overview

Background: NMR is an essential component of structural biology since it is uniquely suited to examine changes in the conformation, structure and mobility of biomolecules using solution conditions closely approximating the biological environment. NMR is critical for chemical analysis and synthetic chemistry, providing atomic “eyesight” for confirming chemical compounds; NMR data is often required for patents and publications. NMR is also a necessary component in drug discovery efforts. Basic NMR applications can be employed for assessing sample purity, chemical changes, binding events and the effects of mutations. More involved multidimensional NMR applications can be implemented for protein structure determination and dynamics investigations.

History: The NMR Core Facility was founded in 1999. The core became part of the WCM Core Laboratories Center (CLC) in 2015.

Location: The NMR Core Facility’s instruments are located in the “S” building annex at 516 East 72nd Street, New York, NY, and in the basement of the Belfer Research Building at 413 East 69th Street, New York, NY.

WCM Core Laboratories Center (CLC): The WCM CLC was established in 2015. The CLC includes core facilities that offer resources and services in genomics and epigenomics, proteomics and metabolomics, flow cytometry, imaging (e.g., optical and electron microscopy, high content screening, MRI, PET/CT, and ultrasound), synthetic and analytical chemistry, and nuclear magnetic resonance (NMR).

Open to all: The resources and services of the core facility are open to all investigators at Weill Cornell Medicine, Cornell University and Cornell-affiliated institutions. The facility also provides services to external investigators at both academic institutions and commercial enterprises.

Services:

Sample preparation: The core offers advice and basic equipment for NMR sample preparation, including solvents, NMR tubes, and instrumentation for preparing protein and small molecule samples.

NMR data collection and data analysis: The core provides state-of-the-art methods for collection and analysis for NMR studies of protein and peptides for analyzing structure, stability and dynamics. Projects can range from rapid 1D analysis of protein structure, or molecular characterization, to measurement of molecular size and protein dynamics, and heteronuclear NMR assignments for investigation of structural change and molecular binding interactions. The core also provides NMR chemical analysis for conformation of chemical composition, characterization of reaction products, investigation of chemical impurities and chemical quantification. We have the capability to rapidly examine arrays of conditions for chemical reactions and protein stability/solubility. The core also offers the capability to test large arrays of chemical reaction products, to confirm the integrity of chemical libraries, and to examine an array of conditions for protein and biological molecule stability studies or for fragment-based drug binding discovery studies.

Consultation and training: The core offers consultation on NMR applications, including project design, sample preparation, data collection methods and data analysis. The core also offers training for unassisted use of the core’s instruments.

Resources

Four NMR spectrometers: Bruker Avance III HD 600 MHz (features include protein NMR applications; cryogenic probe 7000:1 1H S/N). Bruker Avance III HD 500 MHz (features include automated NMR runs; daytime walk up use; small molecular analysis; fragment based drug discovery; protein and peptide studies; cryogenic probe for 1H, 19F, and 13C detection). Bruker Avance III 500 MHz (features small molecular characterization; broad band 1H, 11B, 13C, 19F, 31P detection). Agilent Varian Inova 600 MHz (features include biomolecular NMR applications; cryogenic probe 4000:1 1H S/N)

Links To More Information

For more information about services and pricing, to register as a user of the core, to submit samples, to schedule time on the instruments, and/or to arrange for training, please go to the WCM iLab portal.

Contact Information

W. Clay Bracken, Ph.D.
Core Director, CLC Nuclear Magnetic Resonance (NMR) Core Facility
(212) 746-6473
wcb2001@med.cornell.edu