Genetically Modified Animal Phenotyping

The decoding of the human genome and advancements in scientists' abilities to manipulate the mouse genome have resulted in the generation of countless mouse models of human disease and tools to dissect the function of specific genes. It is expected that the numbers of genetically engineered mice carrying transgenes, targeted mutations, and chemically-induced mutations will only continue to increase.

The Genetically Modified Animal (GMA) Phenotyping Service exists to serve investigators at Memorial Sloan-Kettering Cancer Center, the Rockefeller University, and the Weill Medical College of Cornell University by providing an extensive baseline phenotypic profile of genetically engineered animals. Such a comprehensive baseline characterization will be invaluable to investigators unfamiliar with normal anatomy, histology, physiology and age- or strain-related background lesions of their research species. Evaluating the entire animal, as opposed to a specific tissue or organ system, will help to identify unanticipated phenotypic changes.

Services

Genetically Modified Animal Phenotyping

This includes one or more of the following:

  • Hematology
    • Complete Blood Count
    • White Blood Cell Differential
  • Clinical Chemistry
    • 27 Biochemical Assay
  • Urinalysis
    • Specific Gravity
    • Colorimetric Test for 10
    • Parameters
    • Sediment Analysis
  • Survey Radiographs
    • Lateral and Dorsal-Ventral Views
  • Gross Necropsy
    • Whole Mouse and Parenchymal Organ Weights
  • Bone Marrow Smear Evaluation
  • Extensive Microscopic Evaluation
    • Digital Images of Macroscopic and Microscopic Lesions
    • Electronic Report
    • Interpretive Summary
    • Recommendations for Ancillary Analyses

Other Services

Subcutaneous hemangiosarcomas in a P53 Homozygous knockout mouse. Hematoxylin and eosin, 40X. Courtesy of Krista La Perle, DVM, PhD

Complete histology services including tissue processing, paraffin embedding, cryostat services and staining with routine stains such as hematoxylin & eosin and special histochemical stains are available. Immunohistochemistry, immunofluorescence, and digital slide scanning are available also through the LCP. The GMA Phenotyping Service has an MX-20 Faxitron , which produces extremely high-resolution radiographs of small laboratory animals (mice and rats), as well as excised tissues and paraffin blocks. Specimens can be placed on adjustable shelves to obtain images magnified up to 5x.

Submission Process

Click here to download a PDF of the presubmission form. The form should be filled out completely.. The resulting phenotypic profile will be greatly enhanced by the extent of the experimental details provided, including tissue- or age-specific gene expression and observed/expected phenotypes. The information provided will enable the GMA Phenotyping Service staff to recommend the age, gender and number of mice required to adequately characterize your mice. However, it is typically recommended that at least two male and two female mice of each genotype, approximately 8-12 weeks of age, be submitted alive. Submitters will need to meet with a LCP pathologist prior to any phenotyping projects. Email LCP@mskcc.org to schedule an appointment. Please do not submit animals unless prior arrangements have been made, as the laboratory cannot house live animals.