CLC Flow Cytometry Core Facility

Services

  • Fluorescence activated cell sorting (FACS)
  • Analysis of cell surface markers
  • Analysis of expression of intracellular proteins
  • Separation of cell suspensions into sub-groups or single cells
  • Sort cells to enrich for populations of interest
  • Cell sorting support for single cell genomics
  • Consultation
  • Seminars, workshops and training

Summary

The WCM CLC Flow Cytometry Core Facility offers state-of-the-art fluorescence activated cell sorting (FACS) instruments and services, and expertise in their applications, to the Weill Cornell Medicine (WCM) community and to outside investigators. Services include support for cell counting, cell sorting, biomarker detection, and protein engineering projects. The facility also provides consultation on project design and data analysis, and offers educational workshops, seminars and training.

Overview

History: The Core Laboratories Center (CLC) Flow Cytometry Core Facility was established in 2016 as a merger of a flow core set up on the Weill Cornell Medicine (WCM) campus in 2012 by the WCM Department of Pathology and a bi-institutional flow core set up on the Hospital for Special Surgery (HSS) campus in 2006 as part of a collaboration between WCM and HSS. The CLC flow core represents a continuing and expanded collaboration between WCM and HSS.

Location: The core is located on the 6th floor of the “A” building and the sub-basement level of the “E” building, 1300 York Avenue, New York, NY.

WCM Core Laboratories Center (CLC): The WCM CLC was established in 2015. The CLC includes core facilities that offer resources and services in genomics and epigenomics, proteomics and metabolomics, flow cytometry, imaging (e.g., optical and electron microscopy, high content screening, MRI, PET/CT, and ultrasound), synthetic and analytical chemistry, and nuclear magnetic resonance (NMR).

Services: The WCM CLC Flow Cytometry Core Facility provides full service (i.e., assisted use) for a wide variety of fluorescence activated cell sorting (FACS) applications, including sorting and analysis of cell surface markers, analysis of expression of intracellular proteins and separation of cell suspensions into sub-groups or single cells. Flow cytometry applications are optimized in close collaboration with investigators and with the staff of the CLC genomics and epigenomics, proteomics and metabolomics, and imaging core facilities, to achieve best possible materials for subsequent study. The Flow Core Facility also offers training in all aspects of flow cytometry, including hands-on use of sorters and analyzers. Soon to be implemented: investigators will be able to use selected instrumentation in the core facility 24/7 without staff assistance (i.e., unassisted use) after training and certification by core staff.

Open to all: The resources and services of the core facility are open to all investigators at Weill Cornell Medicine, Cornell University and Cornell-affiliated institutions. The facility also provides services to external investigators at both academic institutions and commercial enterprises.

Resources

Cell sorters: BD Influx: (1) fully enclosed BSL-2 rated hood; (2) 355, 405, 445, 488, 561, and 640 laser lines; (3) 22 fluorescence detectors; (4) sorts cell sizes 1-50 microns; (5) sorts into tubes, plates, and custom collection vessels; (6) 6-way simultaneous sorting (tubes only); and (7) customizable fluidics to accommodate sensitive cells. BD FACSAria II SORP: (1) fully enclosed BSL-2 rated hood; (2) 355, 405, 488, 561, and 640 laser lines; (3) 18 fluorescence detectors; (4) sorts cell sizes 1-35 microns; (5) sorts into tubes, plates, and custom collection vessels; (6) 4-way simultaneous sorting; and (7) unassisted operation for trained core users. Analytical cytometers: BD/Cytek FACSCalibur DxP 11: (1) 405, 488, 561, and 640 laser lines; (2) 11 fluorescence detectors; (3) analysis from single tubes, tube carousel, and 96-well plates; (4) unassisted operation for trained core users. BD FACScan: (1) 488 and 640 laser lines; (2) 4 fluorescence detectors; (3) analysis from single tubes; (4) unassisted operation for trained core users. Data analysis: Site license for FlowJo analysis software.

Links To More Information

For more information about services and pricing, to register as a user of the core, to submit samples, to schedule time on the instruments, and/or to arrange for training, please go to the WCM iLab portal.

Contact Information

Jason McCormick
Co-Manager, WCM CLC Flow Cytometry Core Facility
(212) 746-3387
jam2073@med.cornell.edu

Tomas Baumgartner
Co-Manager, WCM CLC Flow Cytometry Core Facility
(646) 926-7611
tob2008@med.cornell.edu