CLC Proteomics and Metabolomics Core Facility

Overview

The Proteomics and Metabolomics Core Facility at Weill Corenll Meyer Cancer Center was established in 2016. This core provides high quality proteomics and metabolomics services to all investigators at Weill Cornell Medicine, Cornell University and Cornell-affiliated institutions. The facility also provides services to external investigators at both academic institutions and commercial enterprises.

Services

  • Protein Identification: ID of proteins from SDS-PAGE and of proteins in-solution.
  • Protein Quantitation: Label-free (intensity based / spectral counting); stable isotope labeling by amino acids in cell culture (SILAC); tandem mass tags (TMT); other chemical labeling (dimethyl, etc.); spiked standards.
  • Post-Translational Modification Analysis: Phosphorylation; ubiquitination; acetylation; N-term / C-term processing; other PTMs.
  • Metabolomics: Detection of metabolites, drugs, and other small molecules; global profiling (untargeted or discovery); targeted quantitation.
  • RapidFire High Throughput Screening: The RapidFire unit is a micro-scale solid-phase extraction (SPE) based, front-end instrument that enables detection of native compounds by mass spectrometry. Requiring no labels or surrogates, it enables the screening of intractable or challenging targets that are not amenable to fluorescence or luminescence. Sample throughput is 10x faster than conventional LC/MS screening methods.
  • MS Data Analysis: Database searching (protein / metabolite identification); statistical analysis for quantitation; pathway analysis.

Instrumentation

  • Orbitrap Fusion Lumos (Thermo)
  • Orbitrap Q Exactive (Thermo)
  • Triple quadrupole 6495 (Agilent)
  • RapidFire high-throughput SPE system (Agilent)
  • Easy-nLC 1200 UPLC (Thermo)
  • Easy-nLC 1000 UPLC (Thermo)
  • Vanquish UPLC (Thermo)
  • 1290 Infinity II UPLC(Agilent)

To Submit Samples

More information about how to submit samples, is available on the WCM iLab portal.

Service Fees

Click here to download the price list.

Contact Us

Director
Guoan Zhang, Ph.D.
Phone: (646) 962-6222
Email: guz2004@med.cornell.edu

Core staff
Taojunfeng Su, B.S.
Research Specialist
Phone: (646) 962-9739
Email: tas2033@med.cornell.edu

Alexandra Bennett, B.S.
Research Technologist
Phone: (646) 962-9739
Email: aab2007@med.cornell.edu

Shipping Address
413 East 69th Street, BRB-1552G
New York, NY 10021
U.S.A.
Phone: (646) 962-9739